Fig. 4

Production and characterisation of the 5 RANbodies against NDV particles. a IFA detection of the 5 RANbodies expressed in the HEK293T cells. A picture of the NDV-RANbody-49 is shown here, which is same as other 4 RANbodies against NDV. b Western blot analysis of the 5 RANbodies against NDV secreted into the medium of HEK293T cells. The anti-His monoclonal antibody was used as the first antibody and the HRP-goat anti-mouse antibody as the second antibody. Lanes 1–5: NDV-RANbody-2, -4, -24, -30 and -49. c Analysis of the 5 RANbodies specifically binding with the NDV particles with direct ELISA and the anti-H9N2 RANbody as the negative control. d Titers of the 5 RANbodies in the medium of HEK293T cells using direct ELISA detection