Fenobody and RANbody-based sandwich enzyme-linked immunosorbent assay to detect Newcastle disease virus

Table 5 Optimized incubation times of NDV-fenobody-4 capturing antigens and the NDV-RANbody-49 detection antigen with the sandwich ELISA

Incubation times of between the testing samples with NDV-RANbody-49 (min)	Samples	Incubation times of between NDV-fenobody-4 with the testing samples (min)
	Samples	20	40	60	80	100
30	P	0.554	0.786	0.588	1.265	1.222
	N	0.066	0.070	0.074	0.068	0.074
	P/N	8.39	11.23	7.95	18.60	16.51
60	P	0.568	0.987	1.063	1.133	1.311
	N	0.077	0.078	0.074	0.087	0.092
	P/N	7.38	12.65	14.36	13.02	14.25
90	P	1.031	1.254	1.169	1.252	1.296
	N	0.078	0.084	0.082	0.076	0.11
	P/N	13.22	14.93	14.26	16.47	11.78

NDV was the positive (P) control and H9N2 AIV the negative (N) control. Italic represents the best conditions

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