Fenobody and RANbody-based sandwich enzyme-linked immunosorbent assay to detect Newcastle disease virus

Table 6 Comparisons of the developed fenobody and RANbody-based sandwich ELISA with a commercial monoclonal antibody-based sandwich ELISA kit and a commercial immune colloidal gold strip by detecting challenged and clinical chicken tissue samples

Samples	Developed sandwich ELISA	Number	Commericial monoclonal antibody-based sandwich ELISA		Agreement (%)	Kappa value	Commericial immune colloidal gold strip		Agreement (%)	Kappa value
Samples	Developed sandwich ELISA	Number	+	−	Agreement (%)	Kappa value	+	−	Agreement (%)	Kappa value
Challenged chicken	+	132	64	68	80.0	0.523	16	116	65.9	0.146
Challenged chicken	−	208	0	208	80.0		0	208	65.9
Clinical chicken	+	105	43	62	83.1		11	94	74.4
Clinical chicken	−	262	0	262	83.1		0	262	74.4

The 340 samples from chickens contained 170 samples inoculated with NDV and 170 inoculated with PBS (from 3, 4, 5, 7, and 10 days post inoculation)

ISSN: 1477-3155