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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Astaxanthin-loaded polymer-lipid hybrid nanoparticles (ATX-LPN): assessment of potential otoprotective effects

Fig. 4

Cell apoptosis of HEI-OC1 pretreated with ATX, or ATX-LPN (1 and 10 μg/ml) for 4 h, followed by CDDP (60 μM, 24 h). a Western blots were performed with anti-cleaved-caspase 3, anti-cleaved caspase 9, anti-p53, anti-Cyto C, and anti-Bcl-2 antibodies. bf Quantificantion of the western blots in (a). g Flow cytometry was used to measure the rate of apoptosis after different treatments. h Quantification of the data in (g). *p < 0.05, **p < 0.01, ***p < 0.001 as compared with CDDP. #p < 0.05, ##p < 0.01, ###p < 0.001 as compared with ATX (1 μg/ml) + CDDP

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