Table 1 The primers used for PCR
Gene name | Primer (5′ to 3′) | Primer sequence |
|---|---|---|
U6 | Forward | CTCGCTTCGGCAGCACA |
Reverse | AACGCTTCACGAATTTGCGT | |
Runx2 | Forward | CGAATAACAGCACGCTATTAA |
Reverse | GTCGCCAAACAGATTCATCCA | |
ALP | Forward | TAGTGAAGAGACCCAGGCGCT |
Reverse | ATAGGCCTCCTGAAAGCCGA | |
Col I | Forward | CCAGAA GAACTGGTACATCAGCAA |
Reverse | CGCCATACT CGAACTGGAATC | |
GAPDH | Forward | TCATGGGTGTGAACCATGAGAA |
Reverse | GGCATGGACTGTGGTCATGAG | |
Smad5 | Forward | CGGTAGCCAACTGACTTTGAGT |
Reverse | ACCTTGTTTCCAGCCCAACA |