Fig. 5

Antimicrobial properties of the electrospun mats. a Macrographs of the disc diffusion test with the corresponding radius of the zone of inhibition (mean ± SD). bS. aureus and cP. aeruginosa growth reflected as optical density (OD600) over time, of the electrospun CS, CS-5CLO8Q, PA6, PA6-PHMB, core/shell NFs and control. d Fluorescence microscopy assessment via live/dead BacLight Syto9/Propidium iodide (PI) bacterial viability assay, and (e–f) relative percentage of live (green) and dead (red) cells upon exposure to the variant groups of electrospun fibers, quantified from the fluorescent intenstity of the microscopic images. All data are shown as average values ± standard deviations from two independent experiments (n = 6). *core/shell is PA6-PHMB/CS-5CLO8Q