Fig. 5

GO induced lysosomal dysfunction in F98 cells. a F98 cells were exposed to 40, 60 and 80 μg/mL GO for 24 h, and then incubated with 1 μmol/L Lyso-Tracker Red. Representative imagines were photographed under a fluorescent microscope. b, c Flow cytometry was used to further detect Lyso-Tracker Red test for quantitative analysis of fluorescence images. d Determination of cathepsin B activity in F98 cells treated with 40, 60 and 80 μg/mL GO for 24 h. All datas expressed as mean ± SD in three independent experiment results. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. control group