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Fig. 7 | Journal of Nanobiotechnology

Fig. 7

From: The interrupted effect of autophagic flux and lysosomal function induced by graphene oxide in p62-dependent apoptosis of F98 cells

Fig. 7

Verification of P62-mediated cytotoxic response. a, b F98 Cells were exposed to GO (40, 60, 80 μg/mL) with or without treatment of rapamycin (8 nM) for 24 h. The cell viability was analyzed using CCK-8 and LDH kits, respectively. ce F98 cells were treated with 60 μg/mL GO with or without treatment of rapamycin (8 nM) for 24 h to investigate the cell apoptosis by Annexin V-FTIC-PI apoptosis kit. The column on the right exhibited the quantification of flow cytometry results. f Similarly, GO-treated cells were incubated with or without rapamycin for 24 h. The expression of p62 (g), LC3II/I (h), Bax (i), Bcl-2 (j), cleaved 3 (k) were detected by western blots. The column on the right showed the quantification of gray-scale value of straps through Imagine J analysis. All datas expressed as mean ± SD in three independent experiment results. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. control group

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