Fig. 5

The loading efficiency and release kinetics of doxorubicin from the blended silk spheres. a A postloading method was used for Dox incorporation into spheres. The mean and standard deviation of three experiments are shown. Dox was released from b H2.1MS1:DOXMS2, c H2.1MS1:MS2, and d MS1:DOXMS2 particles. The Dox-loaded spheres were resuspended in PBS buffer at pH 7.4, 6 and 4.5 and incubated at 37 °C for 7 days. At the indicated time points, the amount of released drug was measured spectrophotometrically. During the first day, the Dox release was determined after 1, 3, and 6 h of incubation. The experiment was performed in triplicate. * indicates statistical significance with p < 0.05; *** indicates statistical significance with p < 0.001