Fig. 1

Schematic representation of SPION/AGO2/antiHer2-MIR376B (SP-AH/MIR376B) synthesis. a Synthesis of PAA-coated superparamagnetic iron oxide nanoparticles (SP). b Fluorescent labeling and thiolation of anti-HER2 antibody. c Conjugation of recombinant AGO2 protein to PAA coated SPIONs via amide bond generating SP-A and its functionalization with maleimide terminated PEG linker (39.7 Å). d Conjugation of dye labeled and thiolated anti-HER2 antibody to maleimide functional SP-A generating SP-AH. e Loading of MIR376B mimic on SPION/AGO2/antiHer2 (SP-AH) NPs