Fig. 6

SP-AH-mediated delivery of MIR376B into mice blocked autophagy in xenografted breast tumors. aMIR376B levels (qPCR) in xenografted MDA tumors following tail vein i.v. injection of PBS or SP-AH/MIR376B (mean ± SD, n = 6 tumors per treatment condition, ***p < 0.01). bBECN1 mRNA levels (qPCR) in xenografted MDA tumors (mean ± SD, n = 8 tumors per treatment condition, ***p < 0.01). cATG4C mRNA levels (qPCR) in xenografted MDA tumors (mean ± SD, n = 8 tumors per treatment condition, *p < 0.05). d Immunostaining of tissue sections of tumors from PBS- or SP-AH/MIR376B-injected mice using anti-BECN1, anti-ATG4C and anti-LC3 antibodies. e Immunoblot analysis of BECN1, ATG4C and LC3 in the tumor extracts from PBS- or SP-AH/MIR376B-injected mice. ACTB was used as loading control. BECN1/ACTB, ATG4C/ACTB and LC3-II/I band intensity ratios were marked. Scale bar: 25 µm