Fig. 1

Macrophages secrete exosomal miRNAs. a M1 and M2 macrophages transfected with a Cy3-labeled miR-26a-5p mimic were co-cultured with BMSCs in a transwell (membrane pore = 0.4 nm) plate; b Particle size of the vesicles secreted from M1 and M2 macrophages were measured by NanoSight analysis; c Represent picture of the ultrastructure of the two kind of exosomes observed by TEM. Scar bar = 200 nm; d The protein levels of CD 63 and CD 81 in the two kind exosomes; e The two kind of exosomes were marked with red flurescence dye PKH26 and co-cultured with BMSCs, red flurescence represents exosomes in BMSCs, scar bar = 50 µm. Data are mean ± SD of triplicate experiments. *p < 0.05, **p < 0.01, ***p < 0.001