Table 1 miRNAs and mRNA primer sequence
MicroRNAs or gene name | Primer sequence (5′ to 3′) |
|---|---|
mmu-miR-5106—Forward | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCTGCCAA |
mmu-miR-5106—Reverse | TCTGTAGCTCAGTTGGCAGACTC |
mmu-U6—Forward | GCTTCGGCAGCACATATACTAAAAT |
mmu-U6—Reverse | CGCTTCACGAATTTGCGTGTCAT |
mmu-SIK2—Forward | AGAAGCAGUCUCAGCUGCAAGCAU |
mmu-SIK2—Reverse | UAUGCUUGCAGCUGAGACUGCUUC |
mmu-SIK3—Forward | CCACAUGCUGGUGUUAGAUCCAAA |
mmu-SIK3—Reverse | AUUUGGAUCUAACACCAGCAUGUG |
mmu-Col 1a1—Forward | CTGACTGGAAGAGCGGAGAG |
mmu-Col 1a1—Reverse | CGGCTGAGTAGGGAACACAC |
mmu-ALP—Forward | TGACTACCACTCGGGTGAACC |
mmu-ALP—Reverse | TGATATGCGATGTCCTTGCAG |
mmu-OCN—Forward | TTCTGCTCACTCTGCTGACCC |
mmu-OCN—Reverse | CTGATAGCTCGTCACAAGCAGG |
mmu-Runx2—Forward | CGCCACCACTCACTACCACAC |
mmu-Runx2—Reverse | TGGATTTAATAGCGTGCTGCC |
mmu-GAPDH—Forward | AGAGTGTTTCCTCGTCCCG |
mmu-GAPDH—Reverse | CCGTTGAATTTGCCGTGA |