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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Saliva exosomes-derived UBE2O mRNA promotes angiogenesis in cutaneous wounds by targeting SMAD6

Fig. 3

Saliva-Exos enhanced the function of HUVECs. a Saliva-Exos were labeled with PHK26 and added to a HUVEC culture. The presence of a fluorescent signal inside the cells documents that saliva-Exos were taken up by HUVECs. b, c CCK8 and EdU assay were performed after HUVECs were treated with PBS (control), saliva, or saliva-Exos. d The cell cycle was assessed by flow cytometry. e Western blotting was used to evaluate the expression of the cell cycle-related proteins, Cyclin D1 and Cyclin D3. f, g Wound scratch assay and transwell assay were used to evaluate migration of HUVECs after treatment with PBS (Control), saliva, or saliva-Exos. h Representative images of the tube formation assay in HUVECs treated with PBS, saliva, saliva-Exos. i, j Quantitative analyses of total branch length and total number of branching points in the three groups. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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