Fig. 6
From: Saliva exosomes-derived UBE2O mRNA promotes angiogenesis in cutaneous wounds by targeting SMAD6
UBE2O mediates HUVEC function by suppressing the SMAD6/BMP2 pathway. a Network map identifying UBE2O target genes. b Assessment of the expression of SMAD6 and BMP2 by Western blotting. c Expression of SMAD6 and BMP2 in HUVECs after indicated treatments evaluated by Western blotting. d, e Proliferation of HUVECs evaluated by the CCK8 and EdU assays. f Detection of the cell cycle-related proteins cyclin D1 and cyclin D3, by Western blotting. g Flow cytometry analysis of the cell cycle. h, i HUVEC migration evaluated by wound scratch and transwell assay. j Representative images of the tube formation by HUVECs after indicated treatments. k, l Quantitative analysis of total branch length and total number of branching points. *p < 0.05, **p < 0.01, ***p < 0.001