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Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: MicroRNA-421-3p-abundant small extracellular vesicles derived from M2 bone marrow-derived macrophages attenuate apoptosis and promote motor function recovery via inhibition of mTOR in spinal cord injury

Fig. 1

M2 BMDM and sEVs extraction and identification. a Flowchart of the isolation of BMDM. b M2 BMDM showed a typical spindle-type appearance. Scale bar: 20 µm. c Flow cytometry analysis of the macrophage markers on M2 BMDM, macrophage markers (CD11b) and M2 macrophage specificity markers (CD206) are highly expressed (**p < 0.01, ***p < 0.001). d QRT-PCR results of macrophage markers in induced M2 BMDM. e A schematic diagram of the extraction of BMDM-sEVs. f Morphology of BMDM-sEVs was observed by transmission electron microscopy (TEM). Scale bar: 100 nm. g Particle size distribution of BMDM-sEVs was measured by Nanoparticle Tracking Analysis (NTA). h Surface markers of BMDM-sEVs and BMDM was measured by western blotting (CD9: 25 kDa; CD81: 26 kDa; CD63: 26 kDa; Calnexin: 90 kDa)

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Journal of Nanobiotechnology

ISSN: 1477-3155

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