Fig. 5

M2 BMDM-sEVs reduce mTOR protein expression through transfer miR-421-3p. a Bioinformatics analysis was used to predict the potential binding microRNA with mTOR. b Schematic representation of a predicted binding site of miR-421-3p in the 3′UTR of mTOR mRNA, and the mutated mTOR 3′UTR (mTOR 3′UTR-mut). c Dual-Luciferase Reporter System was used to detect luciferase activity. ***p < 0.001. d Neurons were treated with M0-sEVs or M2-sEVs for 24 h, then the relative expression of miR-421-3p was detected by qRT-PCR. e Western blot analysis of mTOR protein expression in neurons. f Quantification of mTOR protein expression in three groups. **p < 0.01