Fig. 6

Upregulation of miR-421-3p reduces mTOR protein expression and enhances neuronal autophagy flux. a A schematic diagram of the extraction of NC-sEVs, Mim-sEVs and Inh-sEVs. b M2 BMDM was treated with miR-NC, mimics and inhibitor, then the relative expression of miR-421-3p was detected by qRT-PCR. c Neurons were treated with NC-sEVs, Mim-sEVs or Inh-sEVs for 24 h, then the relative expression of miR-421-3p was detected by qRT-PCR. d Western blot analysis of mTOR protein expression in treated neurons. e Quantification of mTOR protein expression in three groups. **p < 0.01. f Western blot analysis of autophagy-related protein in treated neurons. g Quantification of autophagy-related protein in each group. **p < 0.01; *p < 0.05