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Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: p62/SQSTM1 accumulation due to degradation inhibition and transcriptional activation plays a critical role in silica nanoparticle-induced airway inflammation via NF-κB activation

Fig. 1

SiNPs induced p62 protein accumulation. a BEAS-2b cells were treated with SiNPs at indicated concentrations for 12 h, followed by the cellular p62 protein expression detection by Western blot. Quantification shown below represents the relative p62/ACTB expression levels. b BEAS-2b cells were exposed to SiNPs at 50 μg/mL for indicated durations. Then the cellular p62 expression were assessed by Western blot. Quantification shown below represents the relative p62/ACTB expression levels. c BEAS-2b cells were exposed to SiNPs at 50 μg/mL for 12 h. The p62 protein accumulation was visualized by indirect immunofluorescence assay. Scale bar: 20 μm. d The p62 protein expression level in mice lung tissue was detected by Western blot. n = 5 for each group. Quantification shown below represents the relative p62/ACTB expression levels. e The p62 protein expression level in mice lung tissue was detected by IHC. n = 5 for each group. Scale bars: 100 μm. All quantitative data are presented as mean ± SEM, **p < 0.01, *p < 0.05

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