Fig. 4

The transcription of p62 gene was activated by SiNPs. a BEAS-2b cells were treated with different concentrations of SiNPs for 12 h, and p62 mRNA levels were determined by qRT-PCR. b The cells were treated with SiNPs at 50 μg/mL for indicated durations, and p62 mRNA levels were determined by qRT-PCR. c BEAS-2b cells were exposed to SiNPs at 50 μg/mL for 12 h in the absence or presence of actinomycin D (2 μg/mL), and the p62 mRNA level was measured by qRT-PCR. d The p62 mRNA degradation in BEAS-2b cells exposed to SiNPs was detected by qRT-PCR. e BEAS-2b cells transfected with p62 promoter luciferase reporter and renilla luciferase internal control were treated with or without 50 μg/mL SiNPs for 12 h. Luciferase activity was then detected and normalized to renilla activity. f, g BEAS-2b cells were treated with or without 50 μg/mL SiNPs for 12 h. ChIP analysis was performed with antibodies against Ac-H3(K9/18) (f) or H3K4me2 (g), then analyzed by qRT-PCR. All quantitative data are presented as mean ± SEM. **p < 0.01, *p < 0.05, NS: no significance