Fig. 5

Nrf2 mediated SiNPs-stimulated p62 transcription activation. a BEAS-2b cells were treated with SiNPs at indicated doses for 12 h, and Nrf2 protein expression levels were detected by Western blot. Quantification shown below represents the relative Nrf2/ACTB expression level. b BEAS-2b cells were treated with SiNPs at 50 μg/mL for 12 h, then Nrf2 expressions in nucleus or cytoplasm were detected by western blot. c BEAS-2b cells treated with or without SiNPs at 50 μg/mL for 12 h. ChIP analysis was performed with antibody against Nrf2 and analyzed by qPCR. d BEAS-2b cells transfected with siRNA targeting Nrf2 or control sequence were treated with or without SiNPs at 50 μg/mL for 12 h, followed by detection of p62 mRNA expression using qRT-PCR. e BEAS-2b cells were treated as indicated in (d), followed by detection of p62 protein expression using western blot. Quantification shown below represents the relative p62/ACTB expression level. All quantitative data are presented as mean ± SEM. **p < 0.01, *p < 0.05