Fig. 6

p62 accumulation mediated SiNPs-stimulated inflammation. a, b BEAS-2b cells were treated with different concentrations of SiNPs for 12 h, then IL-1 (a) and IL-6 (b) mRNA levels were determined by qRT-PCR. c, d BEAS-2b cells were transfected with siRNA targeting p62 or control sequence, then p62 mRNA and protein expressions were detected by qRT-PCR (c) and western blot (d) respectively. Quantification right shows the relative p62/ACTB expression level. e, f BEAS-2b cells transfected with siRNA targeting p62 or control sequence were treated with or without SiNPs at 50 μg/mL for 12 h, followed by detection of IL-1 (e) and IL-6 (f) mRNA expression using qRT-PCR. All quantitative data are presented as mean ± SEM. **p < 0.01