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Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: Real-time monitoring of bacterial biofilms metabolic activity by a redox-reactive nanosensors array

Fig. 1

Microfluidic redox-reactive nanoFET biosensor for extracellular bacterial metabolic analysis. a Silicon wafer chip, with 600 nm thermal oxide layer, which contains 200 potential redox-reactive SiNW FET devices, sharing a common gate. The nanoFETs covered with PDMS microfluidic channel connected via tubing to an Eppendorf tube with a small bacterial media sample mixed with oxidase enzyme. The forming bacterial biofilms of B. subtilis are shown in the left panel. Inset: scanning electron microscope image of single redox-reactive nanoFET consisting of 20 nm p-type SiNW connected to the source and drain electrodes. The nanoFETs chip wire-bonded to the PCB holder, which is connected to the electrical recording system (Additional file 1: Figure S13). b Operation mechanism of the redox-reactive nanoFET biosensor. The redox-reactive nanoFET biosensor reversely reduced or oxidized in the present DEHA or H2O2, respectively. When the redox reactive device is oxidized, the conductivity of the device increased, and AQ moieties are formed on the nanoFET surface (right panel). On the other hand, when the redox reactive device is reduced, the conductivity of the device decreased and DHA moieties are formed on the nanoFET surface (left panel)

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