Fig. 4

a Intracellular localization of UC-PMA-RB,Ce6 in HeLa cells after 3 h of incubation (2.5 nM of NPs). From left to right: Red (RB, Ex. 561, Em 620/60), Green (Ce6, Ex. 405, Em. 725/40), merged Red + Green (yellow color indicated colocalization of RB and Ce6), merged image of bright field (BF) + red + green. b Intracellular colocation of UC-PMA-RB and lysosomes and mitochondria in HeLa cells after 3 h of incubation. From left to right: Red (RB, Ex. 561, Em 620/60), Blue (Lysotracker Blue, Ex. 405, Em. 450/50), Green (Mitotracker Green, Ex. 488, Em. 525/50), merged image of blue + red + green. Purple indicates lysosomes and UCNPs colocalization. Scale bars represent 20 µm