Skip to main content
Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Biodegradable hollow mesoporous organosilica nanotheranostics (HMON) for multi-mode imaging and mild photo-therapeutic-induced mitochondrial damage on gastric cancer

Fig. 5

HGC-27 cells were treated with HMON@CuS/Gd plus NIR irradiation, with or without the addition of NAC. a Total ROS images of HGC-27 cells (left), and statistical graph of ROS content in HGC-27 cells (right), detected by flow cytometry. b MTPs images of HGC-27 cells (left), and statistical graph of MTPs in HGC-27 cells (right), detected by flow cytometry. c MitoROS images of HGC-27 cells (left), and statistical graph of mitoROS content in HGC-27 cells (right), detected by flow cytometry. d Expressions of Cyto C, Bax, Bcl-2 and GAPDH proteins in HGC-27 cells, detected by western blotting. e MPTP images of HGC-27 cells, detected by fluorescence microscope. Scale bar: 100 μm. f Expressions of caspase-9, cleaved caspase-9, caspase-3, cleaved caspase-3, and GAPDH in HGC-27 cells, detected by western blotting. g Cell viabilities of HGC-27 cells, detected by CCK-8 assay. h LDH leakage of HGC-27 cells, detected by LDH assay. after treated with or without NAC, in the presence of HMON@CuS/Gd plus NIR treatment. i Cell apoptosis images of HGC-27 cells (left) and statistical graph of total apoptotic rates (right), detected by flow cytometry. j EdU images of HGC-27 cells (left) and statistical graph of EdU-positive rates (right). Scale bar: 100 μm. Data are shown as mean ± SD, n = 3. *** indicates P < 0.001

Back to article page