Fig. 5

Cysteinated hNET-homing peptides can bind to AuNPs-decorated FRT-nanovehicles in site-directed orientation. a Schematic representation of naked FRT with encapsulated Elli (FRT-Elli, upper), FRT-Elli modified with GASNGINAYLC (middle) and FRT-Elli modified with SLWERLAYGIC (lower). b Size, polydispersity index and ζ-potential. c Transmission electron micrographs. Scale bar, 25 nm. d Colloidal stability for up to 24 h in Ringer’s solution at 20 °C. e Relative expression of SCARA5 in neuroblastoma cell lines. *indicates significant (p < 0.005) difference between SH-SY5Y and UKF-NB-4 cells. f Immunofluorescence of hNET (red) in control neuroblastoma cells and the same cells after 24 h treatment with 10 µM suberanilohydroxamic acid (SAHA). Hoechst 33342 was used to counterstain nuclei (blue). Scale bar, 50 µm. *indicates significantly (p < 0.05) increased hNET expression after SAHA treatment