Fig. 6

hNET-homing nanovehicles are able to induce endocytosis of hNET in SH-SY5Y cells. a Uptake kinetics of 20 µM Elli, FRT-Elli, FRT-Elli-GASNGINAYLC and FRT-Elli-SLWERLAYGIC observed over 24 h revealed by flow cytometry. Aa Elli fluorescence intensity at various time points. Ab The percentage of Elli-positive cells. b Intracellular trafficking of FRT (green) stained with Cy3 NHS ester and encapsulating Elli (80 µM, white), immunofluorescence of hNET (red) and co-localization of FRT and hNET. Hoechst 33342 was used to counterstain nuclei (blue). Scale bar, 10 µm. Manders’ coefficient M1 shows overlapping of hNET on FRT. Manders’ coefficient M2 shows overlapping of FRT on hNET. c Co-localization of FRT (green) encapsulating Elli (white) and immunofluorescence of early endosomal marker Rab5 (red). Hoechst 33342 was used to counterstain nuclei (blue). Scale bar 10 µm. Manders’ coefficient M1 shows overlapping of Rab5 on FRT. Manders’ coefficient M2 shows overlapping of FRT on Rab5. Manders’ coefficients were taken for the representative images only