Fig. 3
From: An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy
Assessment of LDEVs’ effect on gastric cancer cells. a Flow cytometry analysis of cell cycle phases of AGS, BGC-823 and SGC-7901 treated with LDEVs; b CCK-8 assay to evaluate the cell viability of AGS, BGC-823, and SGC-7901 cells treated with different concentration LDEVs; c Western blot analysis the expression of caspase 3 and cleaved caspase 3 proteins in gastric cancer cells; d Flow cytometry analysis the apoptosis of three gastric cancer cells induced by LDEVs; e Plate colony formation assay of AGS, BGC-823, and SGC-7901 cells with or without LDEVs treatment; f Fluorescence images of the live/dead staining 3D cultured AGS, BGC-823, and SGC-7901 cells (Scale bar = 100 μm). These experiments were performed three times