Fig. 1

Neuron-derived exosomes suppressed the activation of M1 microglia. a Neuronal dendrites and axons were identified by MAP2 and somata by NEUN immunostaining. b Representative immunostaining image of IBA1 and INOS in the microglia. c The protein expression levels of INOS were detected by western blot in microglia in the different groups. d Morphology of exosomes under TEM (50–150 nm). e NTA analysis exhibit exosomes size. f Western blot analysis of exosome surface markers. g The red fluorescence dye Dil-labeled exosomes was uptaken into microglia. *P < 0.05