Fig. 4

MiR-124-3p is upregulated in Exos and transferred to microglia by exosomes. a The 156 upregulated and 35 downregulated miRNAs in Heat map with a ≥ 1.5-fold difference between microglia treated with exosomes and PBS. b qRT-PCR was used to compare the top five elevated miRNAs including miR-124-3p, miR-149-3p, miR-218-5p, miR-29c-3p, and miR-31 between microglia treated with exosomes and PBS. c miR-124-3p knockdown and overexpression in neurons and the efficiency was confirmed using qRT-PCR. d The relative expression level of miR-124-3p in exosomes derived from neurons transfected with miR-124-3p^OE, miR-NC^OE, miR-124-3p^KD and miR-NC^KD. e Expression level of miR-124-3p in microglia after administering miR-NC^KD-Exos, miR-124-3p^KD-Exos, miR-NC^OE-Exos and miR-124-3p^OE-Exos. f Representative images of FAM-labeled exosomal miR-124-3p internalized by microglia after administration of miR-NC^KD-Exos, miR-124-3p^KD-Exos, miR-NC^OE-Exos and miR-124-3p^OE-Exos. *P < 0.05, ns indicates no significance