Fig. 4
From: Loop-armed DNA tetrahedron nanoparticles for delivering antisense oligos into bacteria
Td uptake by E. coli with the help of LP2000. a Flow cytometry and b confocal microscopy imaging to analyze the uptake efficiency of Td mixed with LP2000 (LP-Td) by E. coli. The bacterial cells were incubated with different concentrations of FAM-labeled LP-Td (0.1, 0.5, or 1 μM) for 1.5 h and then either treated or not treated with DNase before flow cytometry and confocal microscopy analyses. The LP2000/Td ratio was 0.125 μL/μg. SS: single-strand DNA; Td: DNA tetrahedron; LP: LP2000. SS was used as a control. Scale bars represent 10 μm