Fig. 6
From: Loop-armed DNA tetrahedron nanoparticles for delivering antisense oligos into bacteria
Gene-inhibiting efficiency of LP-Td-L targeting gfp in E. coli expressing GFP. a Confocal microscopy imaging to investigate GFP fluorescence intensity after treating bacteria with 1 μM Td-Lanti-gfp with or without LP2000 for 6 h. Red fluorescence was from FM4-64. b Semiquantitative analysis of GFP fluorescence intensity. c Quantitative real-time PCR analysis of gfp mRNA levels after exposure to different concentrations of Td-Lanti-gfp or LP-Td-Lanti-gfp (0.1, 0.5, or 1 μM) for 3 h. Td-Lmis was used as a negative control to confirm specific gene inhibiting of ASOs. Scale bars represent 10 μm. “*”: p < 0.05; “**”: p < 0.01 versus control group