Table 1 Primers used for real-time PCR
Gene | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) |
|---|---|---|
ALP | AACGTGGCCAAGAACATCATCA | TGTCCATCTCCAGCCGTGTC |
BMP-2 | CAACACCGTGCTCAGCTTCC | TTCCCACTCATTTCTGAAAGTTCC |
COL1 | GCCTCCCAGAACATCACCTA | GCAGGGACTTCTTGAGGTTG |
OCN | GGTGCAGACCTAGCAGACACCA | AGGTAGCGCCGGAGTCTATTCA |
RUNX2 | CCATAACGGTCTTCACAAATCCT | TCTGTCTGTGCCTTCTTGGTTC |
OSX | AAGGCAGTTGGCAATAGTGG | TGAATGGGCTTCTTCCTCAG |
GAPDH | GGCACAGTCAAGGCTGAGAATG | ATGGTGGTGAAGACGCCAGTA |