Fig. 3
From: Dual-responsive doxorubicin-loaded nanomicelles for enhanced cancer therapy
a Evaluation of the ROS regenerating ability of PPD(DMA), PPT/D(DMA) and PPT/D(DMA)@DOX in vitro. Fluorescence images of A549 cells treated with different micelles for different time. Scale bar: 20 μm. b Quantitative analysis the ROS generation in A549 cells by microplate reader. c Cytotoxicity of A549 cells treated with PPT/D(DMA) and PPT/D(SA). d Cytotoxicity of A549 cells treated with free TOS, free DOX and TOS/DOX. e Cell viability of A549 cells treated with PPT/D(SA)@DOX and PPT/D(DMA)@DOX at various concentrations of DOX at pH 7.4 and 6.8. f Apoptosis analysis of A549 cells induced by PBS (control), DOX, TOS, DOX/TOS, PPT/D(SA), PPT/D(DMA), PPT/D(SA)@DOX and PPT/D(DMA)@DOX (equivalent of 2.0 μg/mL DOX and 4.0 μg/mL TOS). g Quantification of apoptosis result. All error bars were presented as mean ± SD. *p < 0.05, **p < 0.01