Fig. 3
The effect of HfO2 on the expression of apoptosis-related genes and miRNAs in MC3T3-E1 and 4B12 cells after 144 h of culture. Expression pattern of CASP9, P53, BCl2 and BAX was determined using RT-qPCR method (A-B). BCL2/BAX ratio was calculated based on relative expression of those genes. GAPDH was used as housekeeping gene. The relative level of miR-7a and miR-17-5p was estimated with Mir-X-system using RT-qPCR method (C-D). U6 was used as normalization control. Error bars represent the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001; Student’s t test