Table 1 Sequence of primers used for mRNA expression analysis using RT-qPCR method
Gene abbreviation | Gene full name | Sequence of primer (5′ → 3′) | Amplicon lenght | Accession No. |
|---|---|---|---|---|
CASP9 | Caspase-9 | F: CCGGTGGACATTGGTTCTGG | 278 | NM_001355176.1 |
R: GCCATCTCCATCAAAGCCGT | ||||
P53 | p53 tumor suppressor | F: AGTCACAGCACATGACGGAGG | 287 | XM_030245924.1 |
R: GGAGTCTTCCAGTGTGATGATGG | ||||
BCl2 | B-cell lymphoma 2 | F: GGATCCAGGATAACGGAGGC | 141 | NM_009741.5 |
R: ATGCACCCAGAGTGATGCAG | ||||
BAX | Bcl-2-like protein 4 | F: AGGACGCATCCACCAAGAAGC | 251 | XM_011250780.3 |
R: GGTTCTGATCAGCTCGGGCA | ||||
TGFB | Transforming growth factor beta 1 | F: GGAGAGCCCTGGATACCAAC | 94 | NM_011577.2 |
R: CAACCCAGGTCCTTCCTAAA | ||||
RUNX2 | Runt-related transcription factor 2 | F: TCCGAAATGCCTCTGCTGTT | 130 | NM_001271630.1 |
R: GCCACTTGGGGAGGATTTGT | ||||
OPN | Osteopontin | F: AGACCATGCAGAGAGCGAG | 340 | NM_001204203.1 |
R: GCCCTTTCCGTTGTTGTCCT | ||||
OCN | Osteocalcin | F: GGTGCAGACCTAGCAGACACCA | 100 | NM_001032298.3 |
R: CGCTGGGCTTGGCATCTGTAA | ||||
c.FOS | c-fos proto-oncogene | F: CCAGTCAAGAGCATCAGCAA | 248 | NM_010234.3 |
R: TAAGTAGTGCAGCCCGGAGT | ||||
MMP9 | Matrix metallopeptidase 9 | F: TTGCCCCTACTGGAAGGTATTAT | 172 | XM_006498861.3 |
R: GAGAATCTCTGAGCAATCCTTGA | ||||
PU.1 | Transcription factor PU.1 | F: GAGAAGCTGATGGCTTGGAG | 175 | XM_017316733.2 |
R: TTGTGCTTGGACGAGAACTG | ||||
RANK | Receptor activator of nuclear factor κ B | F: TTAAGCCAGTGCTTCACGGG | 473 | NM_009399.3 |
R: ACGTAGACCACGATGATGTCGC | ||||
TRAP | Tartrate-resistant acid phosphatase | F: GTCTCTGGGGGACAATTTCTACT | 241 | XM_006509945.3 |
R: GTTTGTACGTGGAATTTTGAAGC | ||||
CR1A | Calcitonin receptor isoform 1a | F: TGCGGCGGGATCCTATAA | 238 | NM_001355192.1 |
R: AGCCAGCAGTTGTCGTTGTA | ||||
INOS | Nitric oxide synthase | F: GACAAGCTGCATGTGACATC | 325 | NM_001313922.1 |
R: GCTGGTAGGTTCCTGTTGTT | ||||
TNFA | Tumor necrosis factor α | F: ACAGAAAGCATGATCCGCGA | 295 | NM_013693.3 |
R: CTTGGTGGTTTGCTACGACG | ||||
IL6 | Interleukin 6 | F:GAGGATACCACTCCCAACAGACC | 141 | NM_001314054.1 |
R:AAGTGCATCATCGTTGTTCATACA | ||||
IL1B | Interleukin 1 beta | F: TGCCACCTTTTGACAGTGATG | 138 | NM_008361.4 |
R: TGATGTGCTGCTGCGAGATT | ||||
L10 | Interleukin 10 | F: ATTTGAATTCCCTGGGTGAGAAG | 75 | NM_010548.2 |
R: CACAGGGGAGAAATCGATGACA | ||||
IL4 | Interleukin 4 | F: GAATGTACCAGGAGCCATAT | 385 | NM_021283.2 |
R: CTCAGTACTACGAGTAATCCA | ||||
GAPDH | Glyceraldehyde 3-phosphate dehydrogenase | F: TGCACCACCAACTGCTTAG | 177 | XM_017321385.2 |
R: GGATGCAGGGATGATGTTC |