Fig. 2
From: Functionalized nanoparticles with targeted antibody to enhance imaging of breast cancer in vivo
Binding and internalization in E0771-E2 cells with either G5-Gd-Trastuzumab-AF647 or G5-Gd-AF647. E0771-E2 cells were incubated with either targeted or control conjugates (conjugate used is labeled above each histogram) and analyzed by flow cytometry. Histograms are presented of E0771-E2 cells incubated with G5-Gd-Trastuzumab-AF647 (left panel), G5-Gd-AF647 (middle panel) or G5-Gd-Trastuzumab-AF647 in an excess of free Trastuzumab (right panel). The histograms show specific uptake of HER-2 positive cells (59.1%) as compared to non-targeted material (8.5%) and targeted material competing with free antibody (12.7%) as detected via the AF647 staining (x-axis). Gates were defined from unstained E0771-E2 cells