Fig. 2

Examples of nanomechanical maps acquired over REF52 (a) and HT1080 (b) cells after the enucleation: the topography (z), apparent YM, and viscoelastic parameters (\(E_{1}\), \(\alpha\), \(\eta\)). A cytoplast and a cell with the retained nucleus are presented on the same map. The phase-contrast image is aligned with the fluorescence of the DNA stain (cyan). Cytoplasts have a lower height as seen on the topography (Z) and a higher stiffness (YM and \(E_{1}\)), while other viscoelastic parameters (\(\alpha\), \(\eta\)) differ less. Scale bars are 20 µm on the optical images and 10 µm on the AFM maps