Fig. 4

The actin cytoskeleton of the cells with the retained nucleus (a, c) and cytoplasts (b, d) of REF52 (a, b) and HT1080 (c, d) cells after the enucleation. Color-coded z-projections of the F-actin staining and reconstructed vertical cross-sections along the marked lines over cells and cytoplasts (green for F-actin, cyan for nucleus staining) are shown. Apical stress fibers that are going over the nucleus (over the nucleus-free cytoplasm in cytoplasts) could be identified in REF52 fibroblasts (b). Scale bars are 10 μm for the z-projections; 5 μm in the horizontal direction and 2 μm in the vertical direction for the cross-sections