Fig. 4

GP-682 micelles enhanced cellular uptake in vitro and improved drug lung targeting in vivo. a BEAS-2B cells treated with Nile Red alone, GP-682/Nile Red micelles, and Nile Red pretreated with 100 μg/mL GP-682 micelles. Fluorescence of Nile Red was observed for 20 min using a confocal microscope. (Data are presented as means ± SD, n = 3, *p < 0.05 compared to the GP-682/Nile Red micelles in 10 min). b Mice were preinjected with or without GP-682 micelles (80 mg/kg) for 30 min, then injected with Lev. The pharmacokinetic parameters of Lev in mouse lung tissue were quantified using HPLC. (Data are presented as means ± SD, n = 5, **p < 0.01 compared to the Lev control group)