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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Interaction kinetics of peptide lipids-mediated gene delivery

Fig. 4

a Transfection efficiency of lipoplexes against HeLa cells. GFP expression of pGFP-N1 was mediated by liposomes LOrn1 and LOrn3 at the charge ratios ( ±) of 1:1, 3:1, 4:1, 6:1 and 8:1. The measurement was carried out in cells by using an inverted fluorescence microscope (10 × 10). b Transfection efficiency in Figure (a) was quantified by flow cytometry analysis. c Agarose gel electrophoresis of liposomes/DNA lipoplexes at various charge ratios. Line M: marker [λ DNA/EcoRI + HindIII]; Line 0: naked DNA [0.2 μg]; Lines 0.5–8: charge ratios were 0.5:1, 1:1, 2:1, 3:1, 4:1, 6:1 and 8:1. d Quantitative analysis by automatic analysis system of gel imaging. e Zeta potential of liposomes/DNA lipoplexes at charge ratios of 1:1, 3:1, 4:1, 6:1 and 8:1. Significance levels: ***p < 0.001

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