Fig. 8
From: Interaction kinetics of peptide lipids-mediated gene delivery
a DNA dissociation profile of the LOrn1/DNA and LOrn3/DNA lipoplexes, evaluated by gel electrophoresis at a pH of 5.5; the lipoplexes were measured at a pH of 7.0 as controls. b Quantitative analysis of accumulative DNA release by gel electrophoresis. Significance levels: *p < 0.05, **p < 0.01. c Intracellular release of DNA in HeLa cells as determined by LSCM. White arrows showed the aggregation state of FAM-labeled DNA. d Co-localization ratio of FAM-DNA (green) and lysosomes (red). 8–10 Cells in each group were selected randomly. Significance levels: *p < 0.05 vs. 12 h, **p < 0.01 vs. 12 h. e Fluorescence spectra of Rhodamine 123 in HeLa cell lysates with culture times of 2, 4, 6, 8, 12, 24, 36, and 48 h after transfection with LOrn3/DNA lipoplex