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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Exosomal PD-L1 and N-cadherin predict pulmonary metastasis progression for osteosarcoma patients

Fig. 5

Effect of exosomes from OS patient serum on the cellular PD-L1, N-cadherin and E-cadherin expression in 143B and U2OS. a Schematic diagram showing the effect of osteosarcoma-derived exosomes on the metastatic lesions; b Nanoparticle tracking system for concentration and size of exosomes derived from medium of 143B and U2OS; c Electron microscopy showing exosomes isolated from medium of 143B and U2OS; d Western blot analysis for the representative biomarkers of exosomes; e The representative TEM image of exosomes immunogold-labelled with anti-PD-L1 antibodies isolated from HOS, KHOS, 143B and U2OS; f Western blot analysis showing PD-L1 expression in four osteosarcoma cell lines and their exosomes; g, h Immunofluorescent analysis detecting internalization of exosomes from 143B cells or OS patient serum to the recipient 143B and U2OS cells; i, j Influence of Sr-exosome isolated from OS patients on PD-L1 expression in 143B and U2OS cells; k–m Influence of Sr-exosome isolated from OS patients on N-cadherin and E-cadherin expression in 143B and U2OS cells

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