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Fig. 7 | Journal of Nanobiotechnology

Fig. 7

From: Exosomal PD-L1 and N-cadherin predict pulmonary metastasis progression for osteosarcoma patients

Fig. 7

Influence of knockdown of Rab27a and PD-L1 on 143B cell. a, b Confirmation of knockdown of 143B Rab27a (143B-Rab27aKD) by CRISPER-Cas9 by qPCR and western blot analysis; c Cell Counting Kit 8 assay showing 143B-Rab27aKD proliferating slower than 143B-shnc; d, e. Representative TEM images showing there was fewer exosomes in the supernate isolated from 143B-Rab27aKD; f, g. Confirmation of knockdown of 143B PD-L1 (143B-PD-L1KD) by CRISPER-Cas9 by qPCR and western blot analysis; h knockdown of 143B PD-L1 exerting no influence on the proliferation; i Comparision of exosomal PD-L1 expression isolated from 143B-PD-L1KD and 143B-WT by immunogold labeling; j Exosomal PD-L1 expression for three osteosarcoma cell lines, including 143B-WT, 143B-Rab27aKD and 143B-PD-L1KD

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