Fig. 8
From: Exosomal PD-L1 and N-cadherin predict pulmonary metastasis progression for osteosarcoma patients
Osteosarcoma-derived exosomes and exosomal-PD-L1 facilitate pulmonary metastasis in metastatic models A. a flowchart showing plan to investigate the effect of osteosarcoma-derived exosomes and exosomal PD-L1 on pulmonary metastasis in vivo; b. Gross specimens of lungs in five groups; c. Representative HE images showing metastasis of 143B-Rab27aKD in BALB/C mice after injection of PBS, exosomes derived from 143B-WT and 143B-Rab27a in Groups A, B and C; d, e. Maximum diameter of metastatic lesion and number of metastasis nodules in three groups; f Effect of exosomes derived from 143B-PD-L1KD in 143B-Rab27aKD model; g–h Maximum diameter of metastatic lesion and number of pulmonary metastasis nodules in C, D and E groups. i, j. Expression of N-cadherin and E-cadherin at the metastatic lesion in Group A, B and C; k, l Influence of exosomes and exosomal PD-L1 on PD-L1 expression of metastatic lesions. Experiments were performed using 5 mice for each group. Data represent mean ± SD