Fig. 2

Characterization of exosomes containing miR-HER2-E1. a Immunoblotting analysis of exosomes and cells with antibodies against the exosome marker proteins Alix, CD9, Annexin V, Flotillin-1 and TSG101. b The particle size distribution and number of isolated exosomes extracted from HEK-293 cells transfected with miR-HER2-E1 or non-targeting (NT) plasmid were measured by Izon’s qNano technology (Izon). c Quantification of miR-HER2-E1 from purified exosomes by qPCR analysis. The amount of exosomal miR-HER2-E1 was quantified and normalized to the amount of 18S rRNA. The data reported are representative of three independent experiments. All quantitative data are presented as the mean ± standard deviation