Skip to main content
Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Exosomes containing miRNAs targeting HER2 synthesis and engineered to adhere to HER2 on tumor cells surface exhibit enhanced antitumor activity

Fig. 3

Inhibition of HER2 protein accumulation by exosome-delivered miR-HER2-E1. Immunoblotting analysis of HER2 protein levels in SK-OV-3 and HEp-2 cells treated with exosome-delivered miR-HER2-E1. a SK-OV-3 cells were left untreated (Con) or incubated with the indicated amounts of exosomes purified from HEK-293 cells transfected with miR-HER2-E1 or nontargeting (NT) miRNA. b The band intensity quantification shown at right represents the relative HER2/GAPDH expression levels in SK-OV-3 cell. c HEp-2 cells were transfected with the HER2 expression plasmid for 36 h and then either left untreated (Con) or incubated with 20 µg of purified exosomes produced by HEK-293 cells transfected with plasmids encoding the miR-HER2-E1 or the NT miRNAs. d The band intensity quantification shown at right represents the relative HER2/GAPDH expression levels in HEp-2 cells. The data reported are representative of three independent experiments. All quantitative data are presented as the mean ± standard deviation. *p < 0.05, N.S. indicates no significant difference

Back to article page