Table 3 Details of the detection results for real sample detection using the electrochemical method and RT-PCR
Sample no | Rct value ± SD (n = 3) | WSSV concentration (DNA copies/ml) | VP28 detection | Shrimp | |
|---|---|---|---|---|---|
by EISa | by RT-PCR | ||||
Control | 2680 ± 146 | 0 | – |  |  |
1 | 7179 ± 238 | 4.8 × 103 | 1.2 × 105 | No | Live |
2 | 2797 ± 72 | 2.4 × 100 | 0 | No | Live |
3 | 8988 ± 108 | 2.0 × 104 | 6.2 × 105 | No | Dead |
4 | 4572 ± 143 | 6.5 × 100 | 0 | No | Live |
5 | 12,101 ± 490 | 2.6 × 105 | 8.4 × 107 | No | Dead |
6 | 16,946 ± 406 | 1.4 × 107 | 9.6 × 108 | Yes | Dead |
7 | 26,949 ± 140 | 4.7 × 1010 | 2.2 × 1010 | Yes | Dead |
8 | 18,946 ± 893 | 6.9 × 107 | 7.5 × 109 | Yes | Dead |
9 | 22,308 ± 195 | 1.0 × 109 | 3.5 × 108 | Yes | Dead |
10 | 13,988 ± 406 | 1.2 × 106 | 2.4 × 108 | Yes | Dead |