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Table 3 Details of the detection results for real sample detection using the electrochemical method and RT-PCR

From: Electrochemical detection of white spot syndrome virus with a silicone rubber disposable electrode composed of graphene quantum dots and gold nanoparticle-embedded polyaniline nanowires

Sample no Rct value ± SD (n = 3) WSSV concentration (DNA copies/ml) VP28 detection Shrimp
by EISa by RT-PCR
Control 2680 ± 146 0   
1 7179 ± 238 4.8 × 103 1.2 × 105 No Live
2 2797 ± 72 2.4 × 100 0 No Live
3 8988 ± 108 2.0 × 104 6.2 × 105 No Dead
4 4572 ± 143 6.5 × 100 0 No Live
5 12,101 ± 490 2.6 × 105 8.4 × 107 No Dead
6 16,946 ± 406 1.4 × 107 9.6 × 108 Yes Dead
7 26,949 ± 140 4.7 × 1010 2.2 × 1010 Yes Dead
8 18,946 ± 893 6.9 × 107 7.5 × 109 Yes Dead
9 22,308 ± 195 1.0 × 109 3.5 × 108 Yes Dead
10 13,988 ± 406 1.2 × 106 2.4 × 108 Yes Dead
  1. aThe copy number of WSSV was determined from the calibration line (Fig. 3C) using the measured Rct value