|
Sample no
|
Rct value ± SD (n = 3)
|
WSSV concentration (DNA copies/ml)
|
VP28 detection
|
Shrimp
|
|---|
|
by EISa
|
by RT-PCR
|
|---|
|
Control
|
2680 ± 146
|
0
|
–
| | |
|
1
|
7179 ± 238
|
4.8 × 103
|
1.2 × 105
|
No
|
Live
|
|
2
|
2797 ± 72
|
2.4 × 100
|
0
|
No
|
Live
|
|
3
|
8988 ± 108
|
2.0 × 104
|
6.2 × 105
|
No
|
Dead
|
|
4
|
4572 ± 143
|
6.5 × 100
|
0
|
No
|
Live
|
|
5
|
12,101 ± 490
|
2.6 × 105
|
8.4 × 107
|
No
|
Dead
|
|
6
|
16,946 ± 406
|
1.4 × 107
|
9.6 × 108
|
Yes
|
Dead
|
|
7
|
26,949 ± 140
|
4.7 × 1010
|
2.2 × 1010
|
Yes
|
Dead
|
|
8
|
18,946 ± 893
|
6.9 × 107
|
7.5 × 109
|
Yes
|
Dead
|
|
9
|
22,308 ± 195
|
1.0 × 109
|
3.5 × 108
|
Yes
|
Dead
|
|
10
|
13,988 ± 406
|
1.2 × 106
|
2.4 × 108
|
Yes
|
Dead
|
- aThe copy number of WSSV was determined from the calibration line (Fig. 3C) using the measured Rct value
