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Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Ni-modified magnetic nanoparticles for affinity purification of His-tagged proteins from the complex matrix of the silkworm fat body

Fig. 6

Binding study of the MNP3s. The capability of 0.6 mg MNPs to bind 10 µg, 50 µg, or 100 µg mCherry. Coomassie blue staining and western blotting results. FT: Flow-through; W1: 1st Washing fraction; W2: 2nd Washing fraction; E1: 1st Elution fraction; E2: 2nd Elution fraction; MNP: MNPs; L1–3: Prepurified loaded samples with the same target protein concentration but different amounts; M: Marker; black arrows indicate the target protein; red boxes indicate the corresponding western blot bands

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