Fig. 6From: Ni-modified magnetic nanoparticles for affinity purification of His-tagged proteins from the complex matrix of the silkworm fat bodyBinding study of the MNP3s. The capability of 0.6 mg MNPs to bind 10 µg, 50 µg, or 100 µg mCherry. Coomassie blue staining and western blotting results. FT: Flow-through; W1: 1st Washing fraction; W2: 2nd Washing fraction; E1: 1st Elution fraction; E2: 2nd Elution fraction; MNP: MNPs; L1–3: Prepurified loaded samples with the same target protein concentration but different amounts; M: Marker; black arrows indicate the target protein; red boxes indicate the corresponding western blot bandsBack to article page