Fig. 4From: Exosomal LncRNA–NEAT1 derived from MIF-treated mesenchymal stem cells protected against doxorubicin-induced cardiac senescence through sponging miR-221-3pExosomeMIF had a better anti-senescent effect in cardiomyocytes treated with Dox. a, b Cell cycle distribution was analyzed. c, d p27 and p16 mRNA levels were analyzed using qRT-PCR. e–h p27 and p16 protein levels were analyzed using western blot analysis. i Representative images of SA-β-gal staining. j Percentage of β-gal-positive cells. k The Telomere length was analyzed by qRT-PCR. l Relative telomerase activity was measured. m, n Representative flow cytometric dot plots of apoptotic cells after Annexin V/propidium iodide staining. *P < 0.05 versus Control; ▲P < 0.05 versus Dox; ○P < 0.05 versus Dox + exosome in repeated measures ANOVA, n = 3Back to article page